Fig. 2.

Monocyte secretomes are adjuvant- and age-specific. A, B, Distribution of proteins determined by LC-MS/MS in the supernatants of differentially treated newborn and adult monocytes. C, Cluster analysis of adjuvant-induced human monocyte secretomes indicates age- and adjuvant-specific differences. Proteins identified in the supernatants from CD14+ monocytes cultured in the presence of Alum (5 μg/ml), MPLA (100 - 1000 ng/ml), or R848 (5 - 50 μm) are shown along the y axis and treatment conditions along the x axis. Neonatal and Adult secretomes clustered separately. The R848-induced secretomes clustered together with the MPLA-secretome and are distinct from the Alum-induced secretome. Color codes reflect the Z scores of the log2-transformed average changes of normalized spectral count (SpC) relative to the untreated control sample. Data are representative of proteins identified in newborn (n = 7) and adult (n = 6) biological replicates, of which six included paired newborn/adult samples.