Fig. 3.

Alum-, MPLA-, and R848-induced human monocyte secretomes in newborns and adults demonstrate age-common and age-specific proteins. Secretome proteins after stimulation of newborn (y axis) and adult (x axis) monocytes with Alum (A, 5 μg/ml), MPLA (B, 100 ng/ml) or R848 (C, 5 μm) are plotted by Z statistic values, reflecting the average change of normalized spectral counts in relation to sterile buffer treatment, taking inter-individual variability into account. Each circle represents one protein. Highlighted in colors are proteins that demonstrated statistically significant enrichment after adjuvant treatment in neonates exclusively (blue), adults exclusively (green) or both neonates and adults (red). Proteins selected for targeted confirmation based on reproducibility of the observed fold change across multiple specimens, novelty and commercial assay availability are labeled. Data represents proteins identified in biological replicates for newborn (n = 7) and adult (n = 6) monocyte secretomes.