Table I. List of potential cathepsin L substrates generated using artificial machine learning prediction with a training dataset for cathepsin L cleavage specificity from MEROPS peptidase database.

Non-prime Sequence	Prime Sequence	UniProt	Protein Name	Average log2 (Ctsl−/−/wt)	Position	Length	Machine learning score
ADIALVELLY	HVEELDPGVVDNFPLLKALR	P30115	Glutathione S-transferase A3;	−10.0	166	221	7.98
FEESFQKALR	MCHPSVDGFTPR	Q8C196	Carbamoyl-phosphate synthase (ammonia), mitochondrial;	−10.0	815	1500	6.85
AEGFKGKILF	IFIDSDHTDNQR	P09103	Protein disulfide-isomerase;	−10.0	291	509	6.81
KSGENFKLLY	DLADQLHAAVGASR	Q99LC5	Electron transfer flavoprotein subunit alpha, mitochondrial;	−10.0	236	333	6.13
AMDGTEGLVR	GQKVLDSGAPIKIPVGPETLGR	P56480	ATP synthase subunit beta, mitochondrial;	−10.0	122	529	5.70
PSPSPSPSLS	STQSAVSKAGAGAVVPKLSHLPR	Q9DC70	NADH dehydrogenase (ubiquinone) iron-sulfur protein 7, mitochondrial;	−10.0	46	224	5.63
DLYTAKGLFR	AAVPSGASTGIYEALELR	P17182	Alpha-enolase;	−10.0	33	434	5.58
EVGALAKVLR	LFEENEINLTHIESR	P16331	Phenylalanine-4-hydroxylase;	−10.0	54	453	4.19
EHPGGEEVLR	EQAGGDATENFEDVGH	P56395	Cytochrome b5;	−10.0	53	134	3.45
EHPGGEEVLR	EQAGGDATENFEDVGHSTDAR	P56395	Cytochrome b5;	−10.0	53	134	3.45
CDVDIRKDLY	ANTVLSGGTTMYPGIADR	P60710	Actin, cytoplasmic 1;	−10.0	295	375	2.55
SLLQQQKTSR	SNMDNMFESYINNLR	P11679	Keratin, type II cytoskeletal 8;	−10.0	140	490	2.40
PFSQHVRRLR	SSITPGTVLIILTGR	P47911	60S ribosomal protein L6;	−10.0	150	296	2.33
DILNMDKTLK	GLNSDSVTEETLR	Q8C196	Carbamoyl-phosphate synthase (ammonia), mitochondrial;	−0.93	893	1500	2.16
LQDCMSKMQR	MVQESSSGGLLDR	Q571F8	Glutaminase liver isoform, mitochondrial;	−10.0	118	602	1.69
DTDDTATALR	EAQEEVGLHPH	Q99P30	Peroxisomal coenzyme A diphosphatase NUDT7;	−10.0	92	236	1.43
TKYPQLLSGIR	GISEETTTGVHNLY	P50247	Adenosylhomocysteinase;	−10.0	152	432	0.83
FMAILCRGID	HTVVYWLGRR	Q3V0D6	Protein 4930544L04Rik	−10.0	26	104	0.70
AVSCLWGKVN	SDEVGGEALGR	P02088	Hemoglobin subunit beta−1;	−1.16	21	147	0.58
AAVAAAREER	GLSPIWAINSPATAEVIR	G3X982	Aldehyde oxidase 3;	−10.0	1291	1335	0.52
GGFLGQRIVR	MLVQEEELQEIR	Q61694	3 beta-hydroxysteroid dehydrogenase type 5;	−10.0	22	373	0.36
KTQDPAKAPN	TPDVLEIEFKKGVPVKVTNIKDGTTR	J3QNG0	Uncharacterized protein	−10.0	219	412	0.25
KTQDPAKAPN	TPDVLEIEFKKGVPVKVTNIKDGTTR	J3QNG0	Uncharacterized protein	−10.0	219	412	0.25
NRRRLSELLR	YHTSQSGDEMTSLSEYVSR	P11499	Heat shock protein HSP 90-beta;	−10.0	457	724	0.17 .te