A 2-D visualization for MYO18A gradient profile and its relation to other
organelle-assigned proteins.
B Gradient profile for MYO18A, MYO18A-associated host proteins (MYH9/10 and
GOLGB1), and markers for the PM and lysosome.
C Gradient profiles for MYO18A, viral proteins with similar profiles, and
lysosome markers.
D IF microscopy of MYO18A and Golgi marker (GOLGB1). Anti-MYO18A antibody detects
both the full and the truncated (lacking the PDZ domain) isoforms. Scale bar
= 10μm.
E Co-staining of MYO18A and UL99 (Assembly Complex marker) by IF under different
permeabilization conditions. Scale bar = 10μm.
F RNAi-mediated knockdown of Myo18A decreases infectious particle production, as
measured by IE1-positive infectious units (IU) per ml. Error bars show
95% C.I. Statistical significance (**) was assessed by
two-sided student t-test for siCtrl vs siMyo18A-1
(p=9.7×10-5) and siCtrl vs siMyo18A-2
(p=8.9×10-4).
G Hypothetical model for MYO18A function in HCMV replication.