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. Author manuscript; available in PMC: 2017 Oct 26.
Published in final edited form as: Cell Syst. 2016 Sep 15;3(4):361–373.e6. doi: 10.1016/j.cels.2016.08.012

Figure 7. MYO18A translocates to the viral Assembly Complex and is important for viral production.

Figure 7

A 2-D visualization for MYO18A gradient profile and its relation to other organelle-assigned proteins.

B Gradient profile for MYO18A, MYO18A-associated host proteins (MYH9/10 and GOLGB1), and markers for the PM and lysosome.

C Gradient profiles for MYO18A, viral proteins with similar profiles, and lysosome markers.

D IF microscopy of MYO18A and Golgi marker (GOLGB1). Anti-MYO18A antibody detects both the full and the truncated (lacking the PDZ domain) isoforms. Scale bar = 10μm.

E Co-staining of MYO18A and UL99 (Assembly Complex marker) by IF under different permeabilization conditions. Scale bar = 10μm.

F RNAi-mediated knockdown of Myo18A decreases infectious particle production, as measured by IE1-positive infectious units (IU) per ml. Error bars show 95% C.I. Statistical significance (**) was assessed by two-sided student t-test for siCtrl vs siMyo18A-1 (p=9.7×10-5) and siCtrl vs siMyo18A-2 (p=8.9×10-4).

G Hypothetical model for MYO18A function in HCMV replication.