Table 1.
Comparison of the Five Most Rapidly Expanding TCR Sequences in HLA-A01+ PBMC Stimulated by HLA-A02 or BKV LTA peptides.
| A02 Stimulation | LTA Stimulation | ||||
|---|---|---|---|---|---|
|
| |||||
| CDR3 sequence | % Clone Frequency | Fold Change | CDR3 sequence | % Clone Frequency | Fold Change |
| CASSSQGDRVQETQYF* | 0.0502 | 2006.5 | CASSSQGDRVQETQYF* | 0.0539 | 1235.0 |
| CASSSGTGDQPQHF | 0.2990 | 1849.1 | CSASQRQSSNQPQHF | 0.1869 | 711.6 |
| CASSLGLSGGQETQYF | 0.2066 | 1283.9 | CASSPTRYEQYF | 0.0796 | 524.9 |
| CASSPTWTGGPYNEQFF | 0.0775 | 688.7 | CASSPWGAHSGNTIYF | 0.1124 | 428.3 |
| CASSRTVSGANVLTF | 0.0566 | 451.2 | CSVDGPGQGAQYF | 0.1081 | 379.8 |
*
The most abundant sequence (first row) is common to both stimulation conditions and indicates that a single T-cell clone can potentially cross recognize HLA-A02 and BKV LTA antigens. The remaining sequences are unique and of potential utility in distinguishing between alloimmune and anti-viral immune responses.