Figure 3. Effects of IFN-γ depletion on NK cell and DSA mediated chronic allograft vasculopathy.

In B6.rag−/−γc−/− recipients bearing C3H allografts that received DSA (α-H2^k IgG2a) and NK cells deficient in IFN-γ production, CAV formation was virtually eliminated (p = 0.0007) in comparison to controls. To test this pathway via an alternate means, B6.rag−/− recipients bearing C3H allografts were treated with DSA in addition to IFN-γ depleting mAb. Treatment with anti-IFN-γ IgG1 also leads to a significant decrease in CAV formation in comparison to controls (p = 0.0411). The control groups as indicated on this figure are the same as depicted in Figure 2 and are provided for comparative purposes. Annotations are as in Figure 2. CAV, chronic allograft vasculopathy; DSA, donor-specific antibody.