TGR5 agonist (INT-777) inhibition of basal secretion induced by several endogenously-released segretagogues in rat distal colon. Influence of INT-777 on Isc in seromuscular peeled preparations pretreated with the indomethacin (10 μM), atropine (10 μM), hexamethonium (100 μM), 5-HT4 antagonist (RS-39604, 10 μM), L-NAME (100 μM), or DMSO (10 μl). Data are means ± SEM, n as indicated in columns. *p<0.05, **p<0.01, ***p<0.001 vs INT-777 treated group, unpaired t test.