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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Sep 15;36(11):2191–2202. doi: 10.1161/ATVBAHA.116.307559

Figure 4. Infiltration of immune cells in the aortas of control and anti-CD20 antibody treated mice.

Figure 4

A, Representative images showing AAA sections stained for F 4/80 (macrophages, brown), Ly-6B.2 (neutrophils, brown) and nuclei (hematoxylin), scale bar 50 µm. B, The immunostained area in A is determined using ImageJ and expressed as percentage of total aortic area. C, D and E, Flow cytometry quantification of CD45+ immune cells, and B1 and B2 cells in spleen and AAA of control or anti-CD20 antibody treated WT mice via IV, (n=4–5). Non-parametric t-test (Mann-Whitney test) was applied to determine significant differences between the groups. Values are expressed as means ± SEM, “*” and “***” indicate p<0.05 and 0.001, respectively. ‘p’ values >0.05 are indicated on the top of the bars.