Fig. 4.

Effects of ginkgolides and bilobalide on the expression of TLR2, TLR4, and MyD88 in BV2 microglia cells after OGD/R. a, b Kinetics of OGD/R-induced increase in the levels of TLR2, TLR4 in BV2 microglia cells. c Western blot analysis of TLR2, TLR4, and MyD88 expression in BV2 microglia cells after 4 h OGD followed by reoxygenation for additional 6 h with 50 μg/ml of GAPIs, GA, GB, GK, and BB treatment. d Concentration-dependent effects of GB treatment on TLR2, TLR4, and MyD88 protein levels in BV2 microglia cells. After BV2 cells exposure to OGD for 4 h, BV2 cells were reoxygenated for either 6 or 6 h followed by treatment with 25, 50, 100 μg/ml GB respectively. GAPDH was used as the loading control. Western blot images are representative of three independent experiments. Each data point is a mean ± SD (n = 3). ^△ P < 0.01, ^△△ P < 0.05, ^△△△ P < 0.001, as compared with corresponding control group; * P < 0.05, ** P < 0.01, *** P < 0.001, as compared with corresponding model group