Fig. 1.

Chaperone activities of various Hsp70 samples. a Substrate-binding activity of Hsp70 samples applied in different concentrations. b Chaperone refolding activity measured with the aid of the luc-assay. “WT”: a highly pure recombinant wild-type human Hsp70 without any modifications; “135”: rhHsp70.135, a wild-type Hsp70 protein with 6xHis tag; “128”: modified rhHsp70.128 with 6xHis tag. (See Fig. S1). All proteins were expressed in E. coli and purified as described in M&M