Fig. 3.

Effect of Hsp70 preparations on the sensitivity of K-562 cells to the action of CTL. wt: a highly pure recombinant wild-type human Hsp70 without any modifications; 135: rhHsp70.135, a wild-type Hsp70 protein with 6xHis tag; 128: modified rhHsp70.128 with 6xHis tag. Standard assay for cytotoxic lymphocytes (CTL) was employed in the study; C3H mouse splenocytes used as effector cells were taken in 50:1 and 100:1 ratio to K-562 target cells. The value of control cytotoxicity (without Hsp70) was subtracted from the data of all three groups