Fig. 5.

Milk from transgenic and control mice was analyzed in denaturing 10 % SDS gel with subsequent blotting and staining using rabbit polyclonal antibody to hHsp70. 1 rhHsp70 from E. coli without histidine tag, 0.1 μg; 2, 3 0.1 μl of whole milk of transgenic mice expressing wt hHSP70; 4 0.1 μl of control whole milk (non-transgenic mouse); 5, 6 0.1 μl of milk of transgenic mice, expressing modified Hsp70 (equivalent of rhHSP70.128)