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. 2016 Aug 26;21(6):1089–1099. doi: 10.1007/s12192-016-0733-1

Fig. 3.

Fig. 3

Flow cytometric apoptosis analysis of MSCs cultured under hypoxia or normoxia for 2 weeks. BM-MSCs derived from five different donors were cultured and maintained under hypoxia or normoxia for 2 weeks. Cell apoptosis was assessed using flow cytometry-based annexin V-FITC and PI. Annexin V staining is indicative of apoptosis; PI staining is indicative of apoptosis and necrosis