Table 2.
TaqMan™ assays used for qPCR and ddPCR.
| Assay name | Target species | gene | Sequences (5′-3′) | Accession number | Amplicon (bp) | |
|---|---|---|---|---|---|---|
| E.coli/Shig_yccT | E. coli/Shigella spp. | yccT | GCAGCGTGGTGGCAAAAa | CP010315 | 56 | This study |
| CGTGACCACCTTGATTGCATb | ||||||
| CGGATACCGGCAAACc | ||||||
| STEC_stx1 | E. coli | stx1 | GGATTTCGTACAACACTGGATGATC | M16625 | 67 | This study |
| GATCAACATCTTCAGCAGTCATTACA | ||||||
| CAGTGGGCGTTCTT | ||||||
| STEC_stx2B | E. coli | stx2 | ACCCCACCGGGCAGTT | X07865 | 59 | This study |
| CGCGCCTGATAGACATCAAG | ||||||
| TTTTGCTGTGGATATACG | ||||||
| STEC_eae | E. coli | eae | GTAACAATGTCAGAGGCGAGTTG | AE005174 | 73 | Cremonesi et al., 2014 |
| CCACCGCTTGCTTTCAGTTTAA | ||||||
| ATTGCAGCCAAATATT | ||||||
| Salmon_invA | Salmonella spp. | invA | TGGAAAGGGAAAGCCAGCTT | M90846 | 68 | This study |
| AATAGCGTCACCTTTGATAAACTTCA | ||||||
| ACGGTTCCTTTGACGGTG | ||||||
| Camp_spp16S | Campylobacter spp. | 16S | TTTTCGGAGCGTAAACTCCTTT | AB587657 | 66 | This study |
| GCCGGTGCTTATTCCTTAGGT | ||||||
| CTTAGGGAAGAATTCTG | ||||||
| Liste spp._prs | Listeria spp. | prs | GGAGGCTGATTATGTCAAACGAGTA | CP002816 | 88 | This study |
| GCAATCTCTTCAGCTAGTTCACGAT | ||||||
| TTGATCCAAAGTTGAAGATT | ||||||
| L.mono_inlA | L. monocytogenes | inlA | TAACAGACACGGTCTCGCAAA | CP013288 | 66 | This study |
| TCCCTAATCTATCCGCCTGAAG | ||||||
| AGATCTAGACCAAGTTACG |
a
Primer forward.
b
Primer reverse.
c
TaqMan_Probe.