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. 2016 Oct 28;9:111. doi: 10.3389/fnmol.2016.00111

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Copyright © 2016 Gilbert, Stebbing, Kuenzel, Murphy, Zacharewicz, Buttgereit, Stokes, Adams and Friedrich.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic analysis of ATP concentration-dependent functional classes in BV2 cells. (A) Representative [Ca²⁺]_i responses of the three classes Oscillation & Peak, Peak and Other measured in BV2 cells in a high-content setting. (B) Fraction of cells exposed to 0 (N = 3 dishes), 1 (N = 10) 10 (N = 10), 100 (N = 11), and 1,000 μM ATP (N = 12), assigned to one of the three classes. The average fraction of cells assigned to the class Other decreases with increasing ATP concentrations, whereas the average fractions of cells assigned to the classes Oscillation & Peak and Peak increase with increasing ATP concentrations.