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. 2016 Sep 20;5(10):2977–2988. doi: 10.1002/cam4.866

Figure 5.

Figure 5

Effects of recombinant canstatin on the expression of vascular endothelial growth factor (VEGF)‐A, VEGF‐C, vascular endothelial growth factor receptors (VEGFR)‐1, VEGFR‐2, and VEGFR‐3 in squamous cell carcinoma (SCC)‐VII‐induced tumors. (A, C) The presence of VEGF‐A, VEGF‐C (A), VEGFR‐1, VEGFR‐2, and VEGFR‐3 (C) in the sections of PBS‐treated or 5 mg/kg/day recombinant canstatin‐treated SCC‐VII‐induced tumors was determined using immunohistochemical analysis. All tumor sections were digitized and images were captured under a 400× objective magnification. Scale bar = 100 μm. (B, D) Immunohistochemical intensities of VEGF‐A, VEGF‐C (B), VEGFR‐1, VEGFR‐2, and VEGFR‐3 (D) from captured images were analyzed using the Image J program and are represented as bar diagrams. The intensities of the VEGF‐A, VEGF‐C, VEGFR‐1, VEGFR‐2, and VEGFR‐3 staining of the PBS‐treated control were established as 100%. Data are presented as mean ± SD of three independent experiments (***P < 0.001).