Table 1.
Qiagen qPCR primers
| Target | Gene | Function | Product ref. |
|---|---|---|---|
| Fatty acid synthase | Fasn | Catalyses the formation of long-chain fatty acids in fatty acid synthesis | QT00149240 |
| Acetyl coA carboxylase | Acaca | Essential role in regulating fatty acid synthesis | QT01554441 |
| Stearoyl-CoA desaturase 1 | Scd1 | Catalyses a rate-limiting step in the synthesis of unsaturated fatty acids. Key enzyme in fatty acid metabolism. | QT00291151 |
| Lipoprotein lipase | Lpl | Hydrolysis of triglycerides into free fatty acids | QT01750469 |
| Hormone sensitive lipase | Lipe | Hydrolyses stored triglycerides to free fatty acids | QT00169057 |
| 3-hydroxy-3-methylglutaryl-CoA reductase | Hmgcr | Rate-controlling enzyme of the mevalonate pathway that produces cholesterol | QT01037848 |
| Sterol regulatory element-binding protein 1 | Srebf1 | Cholesterol biosynthesis and uptake, and fatty acid biosynthesis | QT00167055 |
| Sterol regulatory element-binding protein 2 | Srebf2 | Cholesterol biosynthesis and uptake, and fatty acid biosynthesis | QT01045870 |
| Apolipoprotein E | Apoe | Lipoprotein metabolism and transport. | QT01043889 |
| ATP-binding cassette transporter A1 | Abca1 | Major regulator of cellular cholesterol efflux and phospholipid homoeostasis | QT00165690 |
| ATP-binding cassette transporter G5 | Abcg5 | Cellular cholesterol efflux, promote biliary excretion of sterols. | QT00157752 |
| Insulin receptor substrate 1 | Irs1 | Transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways in insulin signalling | QT00251657 |
| Hexokinase 2 | Hk2 | Phosphorylates glucose to glucose 6-phosphate in the glycolytic pathway | QT00155582 |
| Hexokinase 4 (Glucokinase) | Gck | Phosphorylates glucose to glucose 6-phosphate in the glycolytic pathway | QT00140007 |
| Phosphofructokinase | Pfk | Converts fructose-6-phosphate to fructose-1,6-bisphosphate, one of the most important regulatory enzymes of glycolysis | QT00159754 |
| Carbohydrate-responsive element-binding protein | Chrebp | Activates of several regulatory enzymes of glycolysis and lipogenesis | QT00125335 |
| Glucose transporter 4 | Glut4 | Cellular glucose transport | QT01044946 |
| Glucose-6-phosphate dehydrogenase | G6pdx | Enzyme in the pentose phosphate pathway, often for tissues actively engaged in biosynthesis of fatty acids | QT01748957 |
| Glycogen synthase | Gys | Converts glucose to glycogen for storage, regulating glycogen/glucose levels | QT00162099 |
| Liver X receptor alpha | Nr1h3 | Nuclear receptor transcription factor regulating cholesterol, fatty acid, and glucose homoeostasis | QT00113729 |
| Peroxisome proliferator-activated receptor alpha | Ppara | Transcription factor and major regulator of lipid metabolism | QT00137984 |
| Peroxisome proliferator-activated receptor gamma | Pparg | Regulates fatty acid storage and glucose metabolism | QT00100296 |
| Beta 2 microglobulin | B2m | Reference gene | QT01149547 |
| Glyceraldehyde 3-phosphate dehydrogenase | Gapdh | Reference gene | QT01658692 |