Table 2.
Antibody parameters
| Antibody | Concentration | Diluent | Supplier |
|---|---|---|---|
| FASN | 1:500 | 2.5 % milk bsa in tbs | CST |
| ACACA | 1:500 | 2.5 % milk bsa in tbs | CST |
| ABCA1 | 1:250 | 2.5 % milk bsa in tbs | abcam |
| APOE | 1:250 | 5 % milk in tbs | abcam |
| GCK | 1:500 | 5 % milk in tbs | abcam |
| PFK | 1:250 | 0.01 % milk bsa in tbs | Proteintech |
| GLUT4 | 1:500 | 2.5 % milk bsa in tbs | CST |
| HK2 | 1:500 | 2.5 % milk bsa in tbs | CST |
| LXR | 1:500 | 1 % milk bsa in tbs | abcam |
| G6PD | 1:500 | 0.01 % milk bsa in tbs | Sigma |
| GAPDH | 1:5000 | 2.5 % milk bsa in tbs | abcam |
| Calnexin | 1:1000 | 5 % milk in tbs | CST |
bsa bovine serum albumin, tbs tris-buffered saline, CST cell signalling technologies