FIGURE 1.

Cytotoxic activity of the induced exoproteome of the V. dahliae strain Vd991. (A) Virulence detection of Vd991 infection on cotton. Two-week-old seedlings of susceptible cotton of G. hirsutum L., ‘Junmian No.1’ were inoculated with sterile water (Mock), wild-type (WT) V. dahliae. The disease symptom phenotype was investigated 3 weeks after inoculation. (B) Validation of the cytotoxic activity of the Vd991 exoproteome on cotton leaves. CD medium, C’zapek-Dox medium; CCD medium, cotton-containing C’zapek-Dox medium. Wilting activity of an exoproteome purified from a culture suspension was evaluated on cotton cotyledons. The CD medium and CCD medium were used as the negative controls.