. 2016 Oct 28;7:1677. doi: 10.3389/fmicb.2016.01677

Table 1.

Strains and plasmids.

Strain or plasmid	Genotype or relevant characteristics	Source or references
Escherichia coli DH5	F⁻φ80 dlacZΔM15Δ(lacZYA-argF)U169 endA1 recA1 hsdR17( $r_{K}^{-}$ $m_{K}^{+}$ ) deoR thi-1 supE44 gyrA96 relA1λ⁻	Hanahan, 1985
Escherichia coli HB101	F⁻mcrB mrr hsdS20( $r_{B}^{-}$ $m_{B}^{-}$ ) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(Sm^R) glnV44λ⁻	Hanahan, 1985
Escherichia coli Bl21 (DE3)	huA2 [lon] ompT gal (λ DE3) [dcm] ΔhsdS λ DE3 = λ sBamHIo ΔEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 Δnin5	Novagen
Saccharomyces cerevisiae Y187	MATα ura3-52 his3-200 ade2-101 trp1-901 leu2-3, 112 gal4Δmet⁻ gal80Δ URA::GAL1_UAS-GAL1_TATA-lacZ	Harper et al., 1993
Saccharomyces cerevisiae PJ696	MATα ade2Δtrp1-901 leu2-3,112 ura3-52 his3-200 cyh^R can^R gal4Δgal80Δ met2⁻GAL2::ADE2 GAL1::HIS3 GAL7:lacZ	James et al., 1996
WT	Synechococcus elongatus wild-type	Pasteur culture collection
1Ptrc	WT carrying Ptrc promoter into NSI, Sm^R	Moronta-Barrios et al., 2013
1Ptrc-PlmA	Φ(Ptrc::plmA) NSI, Sm^R	This work
1Ptrc-PlmA PCK1	Φ(Ptrc::plmA) NSI, plmA::C.K1-, Sm^R Km^R	This work
pBridge	TRP1, MCSI GAL4(1-147) BD, MCSII	Clontech
pGAD424	Amp^R, LEU2, GAL4(768-881) AD	Bartel et al., 1993
pGBT9	Amp^R, TRP1, GAL4(1-147) BD	Bartel et al., 1993
pGAD424(+2)	As pGAD424 with a different frame (+2)	Roder et al., 1996
pGBT9(+2)	As pGBT9 with a different frame (+2)	Roder et al., 1996
pBluescript SK+	Cloning vector	Stratagene
pLIC-SGC1	pET expression vector, His₆, Ap^R	Gileadi et al., 2008
pLIC-PII	His-tagged glnB, His₆-PII, Ap^R	This work
pUAGB001	GAL4BD, HA: PipX, Ap^R	This work
pUAGB111	GAL4BD:PII, HA:PipX, Ap^R	This work
pUAGC854	GAL4BD:PlmA, HA:PipX, Ap^R	This work
pUAGC858	GAL4BD:PlmA, HA:PipX²⁻⁷⁰, Ap^R	This work
pUAGC705	GAL4AD:PipX^Y32A, Ap^R	Llácer et al., 2010
pUAGC487	GAL4AD:PipX^R35A, Ap^R	Llácer et al., 2010
pUAGC391	Allele C.S3-pipX^R54C, Sm^R Ap^R	Espinosa et al., 2010
pUAGC390	Allele C.S3-pipX^L65Q, Sm^R Ap^R	Espinosa et al., 2010
pUAGC474	GAL4BD:PipX^E4A, Ap^R	Llácer et al., 2010
pUAGC706	GAL4BD:PipX^Y32A, Ap^R	Llácer et al., 2010
pUAGC488	GAL4BD:PipX^R35A, Ap^R	Llácer et al., 2010
pUAGC498	GAL4BD:PipX^R54C, Ap^R	Llácer et al., 2010
pUAGC372	GAL4BD:PipX^L65Q, Ap^R	Llácer et al., 2010
pUAGC207	GAL4BD:PlmA, HA:PipX^E4A, Ap^R	This work
pUAGC206	GAL4BD:PlmA, HA:PipX^Y32A, Ap^R	This work
pUAGC198	GAL4BD:PlmA, HA:PipX^R35A, Ap^R	This work
pUAGC197	GAL4BD:PlmA, HA:PipX^L65Q, Ap^R	This work
pUAGC199	GAL4BD:PlmA, HA:PipX^R54C, Ap^R	This work
pUAGC208	GAL4BD:PlmA, HA:PipX^L80Q, Ap^R	This work
pUAGC831	GAL4AD:PlmA, Ap^R	This work
pUAGC832	GAL4BD:PlmA, Ap^R	This work
pUAGC709	GAL4AD:PipX²⁻⁷⁰, Ap^R	This work
pUAGC710	GAL4BD:PipX²⁻⁷⁰, Ap^R	This work
pUAGC703	GAL4AD:PipX^L80Q, Ap^R	This work
pUAGC704	GAL4BD:PipX^L80Q, Ap^R	This work
pUAGC6	GAL4AD:NtcA, Ap^R	Espinosa et al., 2010
pUAGC8	GAL4BD:NtcA, Ap^R	Espinosa et al., 2010
pUAGC11	GAL4AD:PII, Ap^R	Burillo et al., 2004
pUAGC12	GAL4BD:PII, Ap^R	Burillo et al., 2004
pUAGC13	GAL4AD:PII^S49A, Ap^R	Burillo et al., 2004
pUAGC15	GAL4AD:PII^S49D, Ap^R	Burillo et al., 2004
pUAGC17	GAL4AD:PII^S49E, Ap^R	Burillo et al., 2004
pUAGC529	GAL4AD:PII^D14A, Ap^R	Llácer et al., 2007
pUAGC531	GAL4AD:PII^I18A, Ap^R	Llácer et al., 2007
pUAGC533	GAL4AD:PII^N22A, Ap^R	Llácer et al., 2007
pUAGC537	GAL4AD:PII^Q39A, Ap^R	Llácer et al., 2007
pUAGC539	GAL4AD:PII^Q42A, Ap^R	Llácer et al., 2007
pUAGC523	GAL4AD:PII^E44A, Ap^R	Llácer et al., 2007
pUAGC543	GAL4AD:PII^R47A, Ap^R	Llácer et al., 2007
pUAGC521	GAL4AD:PII^E85A, Ap^R	Llácer et al., 2007
pUAGC471	GAL4AD:PipX, Ap^R	Espinosa et al., 2010
pUAGC472	GAL4BD:PipX, Ap^R	Espinosa et al., 2010
pUAGC61	GAL4AD:NAGK, Ap^R	Burillo et al., 2004
pUAGC62	GAL4BD:NAGK, Ap^R	Burillo et al., 2004
pUAGC373	GAL4AD:PII^T−loop+7, Ap^R	Espinosa et al., 2010
pUAGC374	GAL4BD:PII^T−loop+7, Ap^R	Espinosa et al., 2010
pRL161	C.K1 Km^R cartridge	Elhai and Wolk, 1988
pUAGC453	C.S3 into pBluescript SK+	Ruiz et al., 2008
pUAGC833	plmA region into pBluescript SK+	This work
pUAGC836	plmA::C.K1 into plmA, Km^R, Ap^R	This work
pUAGC837	plmA::C.K1 into plmA, Km^R, Ap^R	This work
pUAGC835	plmA::C.S3 into plmA, Sm^R, Ap^R	This work
pUAGC839	Ptrc::plmA allele into NSI, Sm^R, Ap^R	This work
pUAGC280	Ptrc promoter into NSI, Sm^R, Ap^R	Moronta-Barrios et al., 2012