Table 1.
Bacterial strains.
| Strain | Relevant Genotype/Description | Reference/Source |
|---|---|---|
| Escherichia coli | ||
| TOP10 | mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 | Invitrogen |
| Streptococcus pyogenes | ||
| HSC5 | Wild-type, M14 serotype | [12] |
| CovRIFD | HSC5 strain with in-frame deleted covR. This strain has a deletion from the leucine codon (L39) to valine codon (V183) in the gene of CovR (228 amino acid long). This is the parental strain for most mutagenesis in this study. | [10] |
| ΩL897_07695 | Strain of CovRIFD with the disruption of the prophage gene L897_07695 | This study |
| CovRIFD:TnCvfA1 & CovRIFD:TnCvfA2 | Strain of CovRIFD with a transposon insertion into cvfA | [10] |
| CovRIFD:TnL897_07695 | Strain of CovRIFD with a transposon insertion into L897_07695 | [10] |
| CovRIFD:TnHasAupIGR | Strain of CovRIFD with a transposon insertion into the intergenic region immediate upstream of hasA | [10] |