Figure 4.

Identification of siRNAs targeting Kifc1 effectively in vitro and in vivo. (A, a₁) Western blot analysis showed the knockdown effect of Kifc1 siRNAs on KIFC1 protein expression at 72 h. ACTB was used as a loading control. (A, a₂) Immunocytochemistry analysis revealed the knockdown effect of Kifc1 siRNA1 on KIFC1 protein expression in the GC-2 cell line. (B, b₁) Trypan blue staining was used as a marker of siRNA injection; 85% of the seminiferous tubules were injected with siRNA. (B, b₂) Western blot analysis of the KIFC1 protein expression in 3-week-old mouse testis 72 h after injection of Kifc1 siRNA1. (B, b₃) Quantification of Western blot analysis. More than 55% of KIFC1 was decreased after the injections. All the data were collected from three independent experiments.