Figure 1.

OprD is downregulated in the presence of Zn independently of CzcRS. (A) Immunoblot analysis of OprD porin on total protein extract of the Pseudomonas aeruginosa WT strain and the ΔczcRS mutant cultivated for 6 h in Luria-Bertani (LB) medium with increasing concentrations of ZnCl₂ as indicated. Blots were exposed to anti-OprD and anti-Hsp70 (loading control) antibodies. OprD quantification was performed using ImageJ software (NIH, Bethesda, MD, USA). OprD band intensity was normalized to Hsp70 intensity and expressed as a percentage of the condition of the absence of Zn; (B) Growth curves of P. aeruginosa WT strain and the ΔczcRS mutant cultivated in LB medium in the absence or presence of 0.5 mM ZnCl₂ (+Zn).