Table A1.

Primers used in this study.

Amplicon	Primer	Sequence (5′ to 3′)	Position	Length
Coding region
oprD	oprD1	ATCTACCGCACAAACGATGAAGG	772	156
	oprD2	GCCGAAGCCGATATAATCAAACG	927
oprF	oprF1	GGTTACTTCCTGACCGACGA	172	664
	oprF2	TCGGTGTTGATGTTGGTGAT	836
hfq	263	GTCAAAAGGGCATTCGCTAC	3	176
	331	AGATCGCGTGCTTGTAAACC	178
hsp70	406	CCACACCGTGATCGTCTATG	561	185
	407	CGCTTTCCTTCTTGAACTCG	745
czcR	czcR1	GTCATCACCCGGACGCAGATCAT	502	153
	czcR2	GTAGCCGACGCCGCGAATGGTAT	654
phrS	449	CAACTGGAGGCCATCAACAT	21	160
	450	CCTTGCGTGCTCTGTGTATC	180
Promoter
poprD	poprD R	CGCAGATGCGACATGCGTCA	−178	101
	poprD L	GGCGCTCCACTTCATCACTT	−77
Primers used for DNA cloning
Hfq:6His	609	GCCGAATTCGCGAAGCTGGAGCCATCTAC	−200	449
	667	GCCGGATCCTCAATGATGATGATGAT GATGAGCGTTGCCCGGCTCGG	249
Hfq insertion verification	314	TGCCGTATACTGTCGCTCAG	PA4945	1390/3534 *
	315	ATTGAACAGGGTCGATTTGC	PA4944

* Without and with aadA insertion cassette.