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. 2016 Oct 3;7(10):83. doi: 10.3390/genes7100083

Figure 2.

Figure 2

Deletion of EZH2 gene downregulated the expression of EZH2 in RKO and HCT116 cells by shRNA. (a) The white and fluorescence microscope pictures of RKO and HCT116 cells transfected respectively with 0.5 μL control-shRNA and EZH2-shRNA of three different sequences, represented as sh#1, sh#2, sh#3 (200×); (b) Relative expression of EZH2 mRNA in four groups of RKO and HCT116 cells (control-shRNA, sh#1, sh#2, sh#3) detected by QRT-PCR. The data were normalized to the level of GAPDH mRNA. Error bars represent SD (n = 3).