Table 2.

qPCR amplification protocols and primer concentrations.

Target Microorganisms	Initial Denaturation	Denaturation	Annealing	Cycles	Fw nM	Rev nM
Bifidobacterium spp. BifTOT F/BifTOT-R	95 °C, 20 s	95 °C–30 s	60 °C–30 s	40	200	300
Lactobacillus spp. LAC-F/LAC-R	95 °C, 20 s	95 °C–30 s	63.5 °C–30 s	40	200	200
Bacteroides fragilis group Bfra-F/Bfra-R	95 °C, 20 s	95 °C–30 s	60 °C–30 s	40	300	300
Enterobacteria Eco-F/Eco-R	95 °C, 20 s	95 °C–30 s	60 °C–30 s	40	400	400
Clostridium cluster I CI-F1/CI-F2	95 °C, 20 s	95 °C–30 s	60 °C–30 s	40	200	200

Fw = Primer Forward, Rev = Primer Reverse.