Table 3.
Sex-linked markers and sequences for eight presence-absence (PA) loci in R. clamitans
| Proportion with restrction fragment sequenced | |||||
|---|---|---|---|---|---|
| Locus | Sequence | Tadpoles | Adults | ||
| Females | Males | Females | Males | ||
| RaclCT014ab | TGCAGTGTCTCTGAGGGTTTACTGGTGATCCAGCGCATG | 0.27 | 0.58 | 0.00 | 0.92 |
| RaclCT015ab | TGCAGCATATGTGCGTACGGTCGGCGGGAAGGGGTTAAGCTATGTCCAGTGCCCTGCATG | 0.00 | 0.58 | 0.09 | 0.94 |
| RaclCT016ab | TGCAGCTCAGTATCTCCGGCCTCTGTCTGTCCTGTCCTTGACAGCATG | 0.00 | 0.62 | 0.04 | 0.92 |
| RaclCT017ab | TGCAGAAGTGCAGTGCATTGCTGTATGATTGGCCAAAGCATG | 0.30 | 0.60 | 0.09 | 0.96 |
| RaclCT018c | TGCAGCAAGAGGTGAAAACAACCGCTGTTGGCAGCATG | 0.00 | 1.00 | 0.04 | 0.89 |
| RaclCT019c | TGCAGTGCTTGAGATGGATCACACAGTGTGATCCATCTCAAAAACTGCGACTGTTGCATG | 0.00 | 1.00 | 0.05 | 0.89 |
| RaclCT020c | TGCAGGCTGCAAAGAAGAAACGAGAAAGCTGCATG | 0.00 | 1.00 | 0.13 | 0.94 |
| RaclCT021c | TGCAGCATTGCAGTGCATTGTTGTCTGATGATTGGGCAAGCATG | 0.00 | 1.00 | 0.04 | 0.94 |
aThese loci were originally identified as sex-linked with adult specimens but not tadpoles
bThese markers are almost entirely unsequenced in male tadpoles from Forest 5. Restriction fragments for these loci were sequenced in all other male tadpoles
cThese loci were originally identified as sex-linked with tadpole specimens but not adults. While perfectly sex-linked in tadpoles, they are less sex-linked in adults