Table 2.
Hematological changes in male rats treated with Cu NPs and Cu MPs following 28 days-repeated oral dose
| Items | Cu NPs (mg/kg/day) | |||
| 0 | 100 | 200 | 400 | |
| No. of rats | 10 | 10 | 10 | 10 |
| RBC (1012/L) | 7.02 ± 0.234a | 6.69 ± 0.388 | 6.17 ± 0.346** | 6.12 ± 0.612** |
| HB (g/dL) | 16.78 ± 1.212 | 15.77 ± 1.668 | 12.70 ± 1.628** | 11.80 ± 1.187** |
| HCT (%) | 43.43 ± 3.435 | 42.50 ± 2.432 | 37.32 ± 3.179** | 36.47 ± 5.747** |
| MCV (fl) | 53.73 ± 4.388 | 50.09 ± 4.618 | 43.63 ± 9.582** | 43.60 ± 4.486** |
| MCH (pg) | 23.47 ± 1.598 | 21.31 ± 2.788 | 19.10 ± 3.306** | 18.92 ± 2.099** |
| MCHC (g/dL) | 34.68 ± 2.770 | 34.57 ± 3.317 | 33.05 ± 4.339 | 28.31 ± 2.198** |
| PLT (109/L) | 1106.2 ± 104.92 | 1212.7 ± 125.88 | 1258.8 ± 166.73* | 1262.4 ± 121.65* |
| RET (%) | 2.3 ± 0.23 | 2.4 ± 0.51 | 2.7 ± 0.48 | 6.1 ± 2.17** |
| WBC (109/L) | 11.2 ± 0.95 | 11.3 ± 2.13 | 8.5 ± 3.03* | 8.3 ± 1.21** |
| NEU (%) | 11.9 ± 0.95 | 11.2 ± 3.45 | 23.9 ± 5.41** | 36.6 ± 8.15** |
| LYM (%) | 82.3 ± 10.73 | 82.3 ± 12.79 | 71.7 ± 13.28 | 52.7 ± 20.17** |
| MON (%) | 2.2 ± 0.39 | 2.2 ± 0.33 | 2.3 ± 0.51 | 3.0 ± 0.59** |
| EOS (%) | 1.7 ± 0.39 | 1.7 ± 0.33 | 1.6 ± 0.53 | 2.0 ± 0.59 |
| BAS (%) | 0.7 ± 0.21 | 0.8 ± 0.18 | 0.7 ± 0.27 | 0.7 ± 0.19 |
| LUC (%) | 0.7 ± 0.13 | 0.7 ± 0.24 | 1.0 ± 0.19* | 1.2 ± 0.25** |
| Items | Cu MPs (mg/kg/day) | |||
| 0 | 100 | 200 | 400 | |
| No. of rats | 10 | 10 | 10 | 10 |
| RBC (1012/L) | 7.04 ± 0.312 | 7.20 ± 0.286 | 7.00 ± 0.403 | 6.96 ± 0.388 |
| HB (g/dL) | 16.65 ± 1.326 | 16.72 ± 1.169 | 15.96 ± 1.563 | 16.12 ± 1.971 |
| HCT (%) | 43.79 ± 4.793 | 43.61 ± 4.201 | 40.16 ± 5.776 | 41.63 ± 3.304 |
| MCV (fl) | 53.81 ± 5.716 | 52.70 ± 6.621 | 52.63 ± 4.184 | 52.34 ± 7.206 |
| MCH (pg) | 23.61 ± 3.392 | 24.32 ± 2.562 | 24.52 ± 1.397 | 22.93 ± 4.257 |
| MCHC (g/dL) | 34.71 ± 3.392 | 29.67 ± 5.561 | 32.09 ± 3.397 | 32.25 ± 4.257 |
| PLT (109/L) | 1179.7 ± 202.53 | 1047.4 ± 218.36 | 1259.8 ± 187.54 | 1236.5 ± 232.71 |
| RET (%) | 2.5 ± 0.58 | 2.6 ± 0.37 | 2.4 ± 0.39 | 2.7 ± 0.44 |
| WBC (109/L) | 11.3 ± 1.49 | 11.9 ± 2.48 | 12.3 ± 3.49 | 11.56 ± 2.23 |
| NEU (%) | 12.2 ± 2.23 | 10.8 ± 3.91 | 13.2 ± 4.45 | 11.9 ± 3.71 |
| LYM (%) | 82.9 ± 13.2 | 85.0 ± 22.47 | 82.7 ± 16.66 | 83.5 ± 13.09 |
| MON (%) | 2.2 ± 0.48 | 2.3 ± 0.31 | 2.5 ± 0.43 | 2.3 ± 0.54 |
| EOS (%) | 1.6 ± 0.42 | 1.5 ± 0.24 | 1.6 ± 0.40 | 1.8 ± 0.48 |
| BAS (%) | 0.7 ± 0.23 | 0.7 ± 0.17 | 0.8 ± 0.13 | 0.7 ± 0.21 |
| LUC (%) | 0.7 ± 0.22 | 0.9 ± 0.29 | 0.9 ± 0.29 | 0.9 ± 0.23 |
Note. RBC red blood cell, HB hemoglobin, HCT hematocrit, MCV mean corpuscular volume, MCH mean corpuscular hemoglobin, MCHC mean corpuscular hemoglobin concentration, PLT platelet, RET reticulocytes, NEU neutrophils, LYM lymphocytes, MON monocytes, EOS eosinophils, BAS basophils, LUC large unstained cells
aValues are presented as mean ± SD
*, ** P < 0.05, P < 0.01 versus vehicle control group