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. 2016 Oct 28;16:234. doi: 10.1186/s12870-016-0914-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Immunolocalization of putative VvPIN4 protein on longitudinal sections of 10 DAF grapevine fruitlets. a Detection of putative VvPIN4 protein in pericarp cells using anti-AtPIN4. b Control with anti-Actin showing diffuse not polarized fluorescence. Background fluorescence observed on sections treated with anti-AtPIN4 (c) and anti-Actin (d) preimmune serum instead of antiserum. Two independent immunolocalization assays with anti-AtPIN4 and anti-AtPIN4 preimmune serum are shown. Red fluorescence is emitted by FM 4-64 membrane stain. Green fluorescence is emitted by secondary antibody conjugated to fluorescent dye. Bars = 30 μm