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. 2016 Oct 28;15:184. doi: 10.1186/s12934-016-0583-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Nucleotide sequence of the 5′ upstream region of the phaC1 gene (P_phaC1RBS). The promoter location, transcriptional start site, and translation initiation codon have been experimentally identified previously [19]. The putative −35 and −10 promoter region are overlined and labeled. An asterisk indicates the transcriptional start site. The translation initiation codon is boxed. The potential ribosome binding site (RBS) is underlined, and the arrow indicates the disrupted RBS sequence in P_phaC1dRBS