Fig. 3.

Structure of gene expression cassettes with the E. coli promoters, P_trcRBS, P_lacUV5RBS, and P_trpRBS. The −35 and −10 promoter regions are overlined and labeled. Asterisks indicate the transcriptional start sites in E. coli. The 19-bp sequences derived from the region upstream of phaC1 are underlined, and the potential ribosome binding sites (RBS) are double-underlined. In their RBS disruptants, P_trcdRBS, P_lacUV5dRBS, and P_trpdRBS, the potential RBS sequence (AGAGAGA) was changed to TCTCTCT