Table 2.
PHBHHx production by phaJ gene expression-controlled strains
| Strain | Dry cell weight (mg/mL) | PHA content (% dry cell weight) | PHA (mg/mL) | Real cell massa (mg/mL) | 3HHx composition (mol%) |
|---|---|---|---|---|---|
| 005dZ126 | 19.1 ± 0.1 | 84.2 ± 0.2 | 16.1 ± 0.1 | 3.0 ± 0.1 | 2.8 ± 0.1 |
| CnC1A | 19.3 ± 0.2 | 84.7 ± 2.0 | 16.4 ± 0.2 | 2.9 ± 0.4 | 5.3 ± 0.1 |
| CnTRPDA | 19.1 ± 0.6 | 84.2 ± 2.7 | 16.1 ± 0.0 | 3.0 ± 0.6 | 7.8 ± 0.0 |
| CnTRPA | 19.1 ± 0.3 | 82.3 ± 1.5 | 15.8 ± 0.0 | 3.4 ± 0.3 | 8.6 ± 0.1 |
| CnUV5DA | 19.2 ± 0.5 | 83.4 ± 0.9 | 16.0 ± 0.3 | 3.2 ± 0.3 | 9.1 ± 0.0 |
| CnUV5A | 18.7 ± 0.8 | 84.0 ± 1.1 | 15.7 ± 0.4 | 3.0 ± 0.3 | 9.9 ± 0.2 |
| CnTRCDA | 19.1 ± 0.5 | 82.1 ± 0.2 | 15.7 ± 0.3 | 3.4 ± 0.1 | 9.6 ± 0.0 |
| CnC1DB | 18.8 ± 0.2 | 85.0 ± 0.6 | 16.0 ± 0.1 | 2.8 ± 0.2 | 5.8 ± 0.2 |
| CnC1B | 19.3 ± 0.5 | 84.2 ± 0.7 | 16.3 ± 0.3 | 3.0 ± 0.2 | 6.7 ± 0.6 |
| CnTRPB | 18.8 ± 0.1 | 84.7 ± 1.3 | 15.9 ± 0.3 | 2.9 ± 0.2 | 10.0 ± 0.1 |
| CnUV5B | 19.0 ± 0.0 | 84.1 ± 1.9 | 16.0 ± 0.3 | 3.0 ± 0.4 | 10.6 ± 0.0 |
| CnTRCB | 18.2 ± 0.4 | 84.0 ± 3.3 | 15.3 ± 1.0 | 2.9 ± 0.5 | 10.7 ± 0.1 |
The cells were cultured in mineral salt medium with 0.129 w/v% (NH4)2SO4 and 1.5 w/v% palm kernel oil for 72 h. Data represent mean ± SD from three experiments performed in triplicate
aReal cell mass = dry cell minus polyhydroxyalkanoates (PHAs)