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. 2016 Oct 28;16:75. doi: 10.1186/s12896-016-0304-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The effect of the endogenous promoter modification on the expression of TGase in S. lividans. a Partially deleting the 5′-end of the TGase promoter region. b Partially deleting the 3′-end of the TGase promoter region. The recombinant S. lividans TK24 were inoculated into 30 mL of medium (which contained 50 μg/mL apramycine) and cultured at 30 °C and 200 rpm for 48 h