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. 2016 Oct 28;16:75. doi: 10.1186/s12896-016-0304-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Codon optimization of S. hygroscopicus WSH03-13 TGase. The Greek numerals (left side) indicated the sequence type of TGase: I: original sequence of S. hygroscopicus TGase ORF; II: Streptomyces preferred gene sequence of the TGase ORF; III: amino acid sequence of the TGase ORF. The grey shadows and “---” indicated the mutated positions and invariant positions. The numbers (right side) indicated the nucleotide sequences