Fig. 1.

Non-heart-beating (NHB) time extension reduces the survival rate with aggravated liver graft injury. Arterialized C57Bl/6-CBF1 liver transplantations were performed with 0 min, 5 min, 10 min, and 20 min of NHB time, and 120 min of cold ischaemic time, respectively. a Cumulative recipient survival rates were analysed. Results of four independent experiments were combined (N = 8 for each group). Log-Rank (Mantel-Cox) test was used for comparison of different survival curves. *P < 0.05, **P < 0.01, ***P < 0.001. Serum b alanine aminotransferase (ALT) and c aspartate aminotransferase (AST) levels from each group were tested at 6 h post-transplantation (N = 6 for each group). d Liver grafts were sectioned for histological examination. Representative images from one experiment are shown at 400× magnification. e Suzuki scores of each section were evaluated by an experienced pathologist in a blinded fashion (N = 6 for each group). b, c, e Results are presented as mean ± SEM for each group. *P < 0.05, **P < 0.01, ***P < 0.001. NS not significant