| SELEX |
Buffers to approximate anticipated application conditions |
Salt concentrations pH, temperature |
| Selection conditions to promote high affinity |
Choose stringency Incubation time |
| Selection conditions to promote high selectivity |
Negative selections against anticipated interfering molecules |
|
| Engineering |
Maximum affinity and selectivity |
Screen aptamer candidates |
| Minimum size |
Determine the 2D structure Identify contact sites on the aptamer Truncate to remove uninvolved NA |
| Adaptation to sensing mode |
Modify with elements such as linkers and fluorophores |
| Optimum affinity, high selectivity and structural stability |
Evaluate effect of strategic changes in sequence of truncated aptamers Chemically modify |
