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. 2016 Oct 24;215(2):231–243. doi: 10.1083/jcb.201602001

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© 2016 Monaco et al.

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Figure 2. — Nuclear calcium controls the expression of activation markers and cytokines in primary human T cells. T cells transfected with expression vectors for CaMBP4.mCherry (CaMBP4) or mCherry either were left unstimulated (ctr) or were stimulated with a combination of antibodies to CD3 and CD28 or with a combination of PMA and ionomycin. Expression of CD25 (A), CD69 (B), IL-2 (C), and IFNγ (D) in T cells was analyzed by flow cytometry with FITC-conjugated antibodies. Dot plots (left) are representative examples of three independent experiments; histograms (right) show the mean percentage of FITC-positive cells (blue dots) from three independent experiments. Error bars represent SEM. Statistically significant differences are indicated with asterisks (*, P < 0.05; **, P < 0.01).