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. 2016 Oct 24;215(2):231–243. doi: 10.1083/jcb.201602001

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© 2016 Monaco et al.

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Figure 3. — Nuclear calcium signaling does not regulate nuclear translocation of NFATc1 in primary human T cells. (A) T cells transfected with expression vectors for CaMBP4.mCherry (CaMBP4) or mCherry were left unstimulated (ctr), were stimulated with a combination of antibodies to CD3 and CD28 in the presence or absence of cyclosporine A (CsA) and FK506, or were stimulated with ionomycin (iono) for 30 min. Cells were stained with antibodies against NFATc1. Nuclei were stained with Hoechst. Bar, 5 µm. (B) Quantitative analysis of NFATc1 nuclear localization (n = 3). Error bars represent SEM.