Figure 1.

Kif5 expression in BMMCs and cKO^Kif5b MC maturation. (A) Relative quantification of Kif5a, Kif5b, and Kif5c transcripts by real-time PCR in BMMCs from WT and cKO^Kif5b mice. Transcript levels for each sample were expressed as a proportion of the mean value for Kif5b. The data are representative of three independent experiments performed in triplicate. (B) Expression of FcεRI and c-Kit on BMMCs from WT and cKO^Kif5b mice, as determined by flow cytometry. (C) Relative quantification of KLC1, KLC2, and KLC3 transcripts by real-time PCR in BMMCs from cKO^Kif5b and WT mice. Transcript levels for each sample were expressed as a proportion of the mean value for KLC1. The data are representative of three independent experiments performed in triplicate. (D) WT and cKO^Kif5b BMMC lysates were separated by SDS-PAGE and were immunoblotted with anti-Kif5b and anti-GAPDH antibodies. The blots are representative of three independent experiments. (E) Toluidine blue staining of MCs in the back skin of WT and cKO^Kif5b mice. Arrows indicate MCs. Bars, 100 µm. (F) The corresponding absolute MC counts per millimeter squared (mean ± SD). Three histological sections per animal were analyzed for WT (n = 3) and cKO^Kif5b (n = 3) mice. (G) Flow cytometry determination of the proportion of c-Kit– and FcεRI-expressing peritoneal MCs obtained from WT (n = 5) and cKO^Kif5b (n = 5) mice. Error bars represent SD. ns, not significant.