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. 2016 Sep 2;107(10):1430–1442. doi: 10.1111/cas.13018

Figure 4.

Figure 4

Association of podocalyxin‐like protein (PODXL) with gelsolin. (a) Immunoprecipitation of PODXL from S2‐013 pancreatic cancer cells cultured on fibronectin. Proteins within immunoprecipitates (IP) were examined on Western blots probed with anti‐PODXL antibody (left panels). Proteins in immunoprecipitates were examined by silver stain analysis (right panels). The box depicts the position of the section enlarged. Rabbit IgG isotype control antibody was used as an isotype control. Arrow indicates an 80‐kDa band. (b) Immunoprecipitation of PODXL or gelsolin from S2‐013 cells cultured on fibronectin. Proteins within immunoprecipitates were examined on Western blots probed with antibodies against PODXL and gelsolin. Rabbit IgG isotype control antibody for PODXL and mouse IgG isotype control antibody for gelsolin were used as isotype controls. (c) Confocal immunofluorescence microscopic images. S2‐013 (upper panels) and PANC‐1 (lower panels) cells were cultured on fibronectin and then labeled with anti‐PODXL (green) and anti‐gelsolin (red) antibodies. Actin filaments were labeled by phalloidin (violet). Arrows, PODXL colocalized with gelsolin in cell protrusions. Blue, DAPI staining. Bar = 10 μm. IB, immunoblot.