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. 2016 Sep 4;107(10):1390–1398. doi: 10.1111/cas.13016

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© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Effects of galectin‐1 (Gal‐1) knockdown on the malignant characteristics of urinary bladder urothelial carcinoma T24 and J82 cells. (a) Cell survival assays of T24 cell clones with knockdown at +120 nt (sh‐Gal‐1(+120)) and +380 nt (sh‐Gal‐1(+380)). (b) Cell number counting assays of sh‐Gal‐1(+120) T24 cells. (c–e) Soft agar (c), cell migration (d), and cell invasion (e) assays of sh‐Gal‐1(+120) and sh‐Gal‐1(+380) T24 cells. (f,g) Cell migration (f) and cell invasion (g) assays of sh‐Gal‐1(+120) J82 cells. The results of MTT, cell number counting, soft agar, migration, and invasion assays show representative data of three independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.