Table 1.
Construct | Mass (kDa) |
|||
---|---|---|---|---|
Theoreticala | Analytical gel filtrationb |
Sedimentation velocity AUCc |
Equilibrium AUCd |
|
WT | 104 | 163 | 95.8 | 130 ± 6 |
L-Di | 216 | 447 | 219 | 236 ± 2 |
L-Tri | 324 | 633 | 307 | 375 ± 31 |
L-Tet | 436 | 734 | 320 | 517 ± 30 |
Mass estimated from the protein sequence and masses of the cofactors.
Mass estimated using a calibration curve based on gel filtration standards.
Mass estimated from sedimentation coefficients in Sedfit.
Mass determined by curve fitting in Ultrascan II.