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. 2016 Dec;1857(12):1829–1839. doi: 10.1016/j.bbabio.2016.09.002

Table 1.

Mass estimates for PufL-modified oligomeric reaction centres.

Construct Mass (kDa)
Theoreticala Analytical
gel filtrationb
Sedimentation
velocity AUCc
Equilibrium
AUCd
WT 104 163 95.8 130 ± 6
L-Di 216 447 219 236 ± 2
L-Tri 324 633 307 375 ± 31
L-Tet 436 734 320 517 ± 30
a

Mass estimated from the protein sequence and masses of the cofactors.

b

Mass estimated using a calibration curve based on gel filtration standards.

c

Mass estimated from sedimentation coefficients in Sedfit.

d

Mass determined by curve fitting in Ultrascan II.