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. 2016 May 3;590(9):1291–1303. doi: 10.1002/1873-3468.12180

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© 2016 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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The specificity of antisense morpholino oligonucleotides for xWDR26 and siRNA for hWDR26. (A) Western blotting analysis of injected embryos at stage 10. xWDR26‐MO inhibited the translation of FLAG‐tagged mRNA containing the targeted site. The translation of FLAG‐tagged mRNA containing five‐mismatched sequences at the targeted site was not inhibited by xWDR26‐MO. β‐globin is used as loading control. (B) Quantitative RT‐PCR analysis. Three siRNA against hWDR26 were transfected into HEK 293T cells. GAPDH was used as a loading control. The value obtained for each si‐hWDR26 RNA was normalized to the level of GAPDH. The value of transfection of si‐Control RNA (lane 1) was set to 100 and other values were computed. Error bars represent standard deviation of the mean in three experiments. Statistical significances of hWDR26/hGAPDH between si‐Control RNA transfection and si‐hWDR26 RNA transfection were determined by Mann–Whitney U test. P < 0.01 (between lane 1 and each other lane).