Figure 3.

Nucleotide exchange mechanism in EF‐Tu. Schematic of the nucleotide exchange pathway catalyzed by EF‐Ts. Binding of EF‐Ts to EF‐Tu–GDP causes dissociation of the Mg²⁺ ion, which initiates GDP dissociation. GTP is then loaded due to its higher cellular concentration compared to GDP. The change in the conformation into the GTP‐bound form occurs upon rebinding of Mg²⁺ and causes the dissociation of EF‐Ts.23, 40