Figure 4.

Models for GTP hydrolysis of EF‐Tu on the ribosome. (a) General base mechanism; (b) substrate assisted pathway; (c) Two‐dimensional More O'Ferral‐Jencks plot for an A_ND_N reaction. The reaction coordinate proceeds from the lower left (ground state, GS) to the upper right corner (product state, PS). Bond cleavage and bond formation occur along the x and y axes, respectively. The TS can be located anywhere in the area, indicating that GTP hydrolysis can proceed via a continuum of reaction pathways. The farthermost cases of A_N + D_N and D_N + A_N will have additional intermediates in the upper left and lower right corners, respectively. Adapted from Ref. 65.