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EF‐Tu function during decoding on the ribosome. EF‐Tu‐dependent selection of cognate aa‐tRNA and rejection of aa‐tRNAs that do not match the A‐site codon is achieved in two selection steps separated by GTP hydrolysis by EF‐Tu. Shown and individual kinetically‐resolved steps identified by rapid kinetics and smFRET approaches. Adapted from Ref. 112.
