Table 1.

Binding of Different trGTPases to GTP and GDP

	GTP			GDP			GEF	T °C	GTPase	Ref
trGTPase	k on (M−1 s−1)	k off (s−1)	K d (M)	k on (M−1 s−1)	k off (s−1)	K d (M)			k GTP s−1
IF2	4 × 105	15	3.8 × 10−5‐ 7.1 × 10−6			1.4 × 10−6	−	20°	33	128,129
EF‐Tu	5.0 × 105	0.03	6.0 × 10−8	2 × 106	0.002	1 × 10−9	EF‐Ts	20°	>500	130
EF‐G	5.8 × 105	13	2.2 × 10−5			4.0 × 10−5	−	37°	250	45
SelB	1.7 × 105	0.1	6 × 10−7	1.8 × 106	15	8.3 × 10−6	−	20°		131,132
RF3	1.2 × 109	24a	2.0 × 10−8	2.7 × 107	0.13	5 × 10−9	−	37°	0.15	41
eIF2	>5.8 × 105	> 0.1	1.7 × 10−6	1.6 × 105	0.2	2.0 × 10−8	eIF2B	26°		119
eIF5B	1.5 × 106	22	1.5 × 10−5	4.3 × 106	9.6	2.3 × 10−6	−	25°		133
eEF1A	9 × 104	0.1	1.1 × 10−6	3.3 × 105	0.13	4 × 10−4	eEF1B	20°		24
eEFSec			1.1 × 10−7			3.3 × 10−7	−	30°		134
eEF2			3 × 10‐6			2.5 × 10‐6		25°		135
eRF3	7 × 105	13	2.3 × 10−5	3.1 × 106	2.4	8 × 10−7	−	25°		136

^aMeasured with GDPNP.