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. 2016 May 11;75(6):678–693. doi: 10.1111/aji.12512

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© 2016 The Authors. American Journal of Reproductive Immunology Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Sample Reproducibility: Standard curve for analytes was generated by two separate laboratories at different times using the same batch of panels. Representative curves for four analytes. (B) Standard Curve Reproducibility: Stability and reproducibility of the assay was established by generating 11 individual standard curves for human IL‐6 run over the course of 31 days. (C) Intraassay Precision: Intraassay precision was determined for each analyte (IL‐10, IL‐1β, IL‐6, and IL‐5) by running cartridges containing eight replicates of either high or low QCs over a period of 4 days.