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. 2016 May 11;75(6):678–693. doi: 10.1111/aji.12512

Figure 5.

Figure 5

Efficacy of the Simple Plex Platform for the Analysis of Human and Murine Cellular Samples. (A) Detection of human cytokines and chemokines. Detection of circulating TNF‐α levels in serum samples revealing some differential expression levels between pre‐ and post‐menopausal women as well as between healthy post‐menopausal women and ovarian cancer patients. (B) Detection of cytokines and chemokines in peripheral blood monocytes stimulated with TLR4 ligand LPS. CD14 + peripheral monocytes were treated with or without LPS (10 ng/ml) for 24 hrs after which both protein expression and secretion were analyzed using the Simple Plex platform. Detection of TNF‐α, IL‐6, and IP‐10 (CXCL10) were all highly induced and secreted after LPS stimulation, while IL‐1β was only detected in the lysate. (C) Detection of murine cytokines and chemokines. Baseline peritoneal macrophage supernatant and spleen lysate were analyzed using the Simple Plex platform. Murine pro‐inflammatory cytokines TNF‐α, IL‐6, CCL2, and CXCL2 were all detected in the supernatant as well as cell lysate.