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. 2016 Mar 10;27(11):3278–3284. doi: 10.1681/ASN.2014101044

Figure 1.

Figure 1.

Figure 1.

S3 in VH CDR3 of IgG autoantibodies is not encoded in VH germline gene sequences. Alignments of the 3′ termini of VH segments for germline genomic DNA (GG DNA) with reverse-transcribed mRNA from individual IgG-producing clones from seven patients with IgAN (A) (#2047, #1123, #1125, #1139, #1023, #3061, and #3081; red) and six healthy controls (B) (#9017, #3066, #3064, #3070, #8043, and #9035 blue) (cDNA). cDNA sequences were published.6 VH 3′ segment consists of coding 3′ terminus of framework region 3 (FR3) and 5′ part of complementarity-determining region 3 (CDR3), followed by noncoding region sequence composed of conserved 7-mer (CACAGTG; in red) and 5′ terminus of 23-bp spacer nucleotides (recombination signal sequence [RSS]). Chromatograms show GG DNA sequence in rectangles; the triplet-encoding alanine (A) is marked by stars. For cDNA, a coding region of DH segment contributing to CDR3 is shown. In the diagram above the sequences, green marks coding sequences and red marks noncoding sequences. Deduced aa sequences for GG DNA (GG-deduced aa) and cDNA (cDNA aa) are shown to localize the critical A (or V in subject 3061) to serine (S) changes in CDR3 of antibodies from patients with IgAN.